Colloids Surf B Biointerfaces. 2017 Jul 1; 155: 530-537.

Silica nanoparticles as sources of silicic acid favoring wound healing in vitro.

Quignard S, Coradin T, Powell JJ, Jugdaohsingh R.

1Sorbonne Universités, UPMC Univ. Paris 06, CNRS, Collège de France, UMR 7574, Laboratoire de Chimie de la Matière Condensée de Paris, F-75005 Paris, France; Biomineral Research Group, MRC Elsie Widdowson Laboratory, Cambridge CB1 9NL, UK. Electronic address: sandrine.quignard@ens-paris-saclay.fr.

2Sorbonne Universités, UPMC Univ. Paris 06, CNRS, Collège de France, UMR 7574, Laboratoire de Chimie de la Matière Condensée de Paris, F-75005 Paris, France.

3Biomineral Research Group, MRC Elsie Widdowson Laboratory, Cambridge CB1 9NL, UK; Biomineral Research Group, Department of Veterinary Medicine, University of Cambridge, Madingley Rd, Cambridge CB3 0ES, UK.

 

Abstract

There is good evidence that certain silicon-containing materials promote would healing and their common feature is the delivery of orthosilicic acid (Si(OH)4) either directly or following metabolism. In this respect, amorphous silica nanoparticles (NP), which dissolve in aqueous environments releasing up to 2 mM orthosilicic acid, may be appropriate 'slow release' vehicles for bioactive silicon. Here we studied the impact of silica NP suspensions (primary particles∼10 nm) in undersaturated conditions (below 2 mM Si) with differing degrees of surface charge and dissolution rate on human dermal fibroblasts (CCD-25SK cells) viability, proliferation and migration in a cellular wound model. Silica was shown to be non-toxic for all forms and concentrations tested and whilst the anticipated stimulatory effect of orthosilicic acid was observed, the silica NPs also stimulated fibroblast proliferation and migration. In particular, the amine-functionalized particles promoted wound closure more rapidly than soluble orthosilicic acid alone. We suggest that this effect is related to easy cellular internalization of these particles followed by their intracellular dissolution(溶解；融化) releasing silicic acid at a faster rate than its direct uptake from the medium. Our findings indicate that amorphous silica-based NPs may favour the delivery and release of bioactive silicic acid to cells, promoting wound healing

 

Silica NP可以刺激纖維細胞增生與移行。特別的是，有胺基功能化的顆粒促進傷口癒合會比可溶性正矽酸來得快速。我們認為這個效應可能和這些NP顆粒容易被細胞中和(cellular internalization)，進而在細胞內顆粒崩解而快速釋出矽酸有關，這些過程是比直接由培養液uptake矽酸來得快。故，我們的發現指出NP顆粒適合生物活性矽酸釋出的運輸系統而可促進傷口癒合。